Relationships between peripheral circulating tumor cells in patients with gallbladder carcinoma detected by a novel nano microfluidic chip with clinicopathology and prognosis

Abstract

Objective To establish a novel method using a nano microfluidic chip to detect circulating tumor cells (CTCs) in peripheral blood in gallbladder carcinoma, and to study the relationship between CTCs with clinicopathology and prognosis in these patients. Methods The peripheral blood samples of 51 patients with gallbladder carcinoma were collected from June 2014 to January 2017. The CTCs from peripheral blood samples were detected by a novel nano microfluidic chip. This study aimed to study the correlation between CTCs with the clinical and pathological features. The significance of CTCs on prognosis in patients with gallbladder carcinoma was also analyzed. Results The positive rate of CTCs in the peripheral blood of gallbladder carcinoma patients was 43.1% (22/51). There were significant correlations between CTCs with liver metastasis (P 0.05). Conclusions Peripheral blood CTCs in patients with gallbladder carcinoma were efficaciously detected by a novel nano microfluidic chip. Peripheral blood CTCs was closely related to the Nevin staging and liver metastasis. CTCs could serve as a potential prognostic indicator in patients with gallbladder carcinoma. Key words: Gallbladder carcinoma; Microfluidic chip, nano; Circulating tumor cells (CTCs); Prognosis