Relationships between body weight of overwintering larvae and supercooling capacity; diapause intensity and post-diapause reproductive potential in Chilo suppressalis Walker

Abstract

The rice stem borer, Chilo suppressalis Walker, overwinters in China as a larva in facultative diapause. The instars and body weights of overwintering larvae vary widely. In this paper, the relationships between body weight and supercooling capacity, diapause intensity and post-diapause reproductive potential of overwintering larvae collected in late-stage rice field were examined. There was a significant positive correlation between body weight, instar, and head capsule width, thus the overwintering larvae were divided into five groups based on body weight (I, up to 35.0 mg; II, 35.1–57.0 mg; III, 57.1–79.0 mg; IV, 79.1–101.0 mg; and V, over 101.1 mg) for further analysis. The body water content of the lighter group (I) was significantly higher than that of the heavier groups (IV–V). However, the mean supercooling point decreased with an increase of the mean larval body weight in five groups; mean supercooling point of group I was significantly lower than that of group V, except in January 2009. After transfer of overwintering larvae to 15, 20 and 25 °C on different dates, smaller individuals pupated slightly faster than larger ones at the same temperature, suggesting that diapause was less intense in smaller overwintering larvae. On 19 March 2009 there was a strong positive correlation between larval body weight and the weight of 3 day-old pupae, and the number of eggs carried by 2 day-old adult females at 15, 20 and 25 °C. The average number of eggs carried by 2 day-old adult females differed significantly among different groups. The average number of eggs carried by 2 day-old adult females in group V was significantly greater than those of other groups, and that of group I was significantly lower than those of other groups, suggesting that post-diapause reproductive potential was determined, to a certain extent, by body weight of the overwintering larvae.