Relationship of thiol/disulphide homeostasis with oxidative stress parameters in non-diabetic, prediabetic and type 2 diabetic Turkish women

Abstract

Background and Aims: Deterioration of thiol/disulphide homeostasis (TDH) is found in several diseases, including diabetes. This study aimed to examine the association between TDH and oxidative stress parameters in nondiabetics, prediabetics and newly diagnosed type 2 diabetics. Methods: A total of 26 non-diabetic, 24 prediabetic and 19 type 2 diabetic women were involved in our study. They all applied to Zonguldak Bulent Ecevit University, Health Practice and Research Center, Endocrinology and Metabolism Diseases, Diabetes Polyclinic to be tested for type 2 diabetes mellitus. The demographic and laboratory data were collected from the patient files. Oxidative stress parameters and dynamic TDH were investigated using ELISA kits. Results: Total oxidant status (TOS), total thiol and disulphide levels were significantly higher in type 2 diabetics than the non-diabetics (24.24±14.93 versus 14.14±12.19, 646.47±75.51 versus 470.88±180.85, and 179.32±51.24 versus 91.85±40.29, respectively). In type 2 diabetics, a positive correlation between TOS and native thiol, total thiol and disulphide levels was found (P=0.000). In prediabetics, a significant positive correlation was found between total antioxidant capacity and total thiol levels (P<0.05), and also between arylesterase and native and total thiol levels (P<0.05). Conclusion: The elevation of oxidative stress and the deterioration of TDH might cause the formation of symptoms related to high blood glucose levels in type 2 diabetics.