Relationship of serum haemojuvelin and hepcidin levels with iron level and erythropoietin requirement in prevalent hepatitis C virus positive haemodialysis patients.

Abstract

Iron overload is frequently reported in haemodialysis (HD) patients particularly those with chronic hepatitis C virus (HCV) infection. Soluble haemojuvelin (sHJV) has recently emerged as one of the significant regulators of iron homeostasis and hepcidin expression. The aim of the present study was to evaluate the potential associations of sHJV and hepcidin with inflammation, iron parameters and erythropoietin requirement in prevalent HD patients with HCV. Serum sHJV and hepcidin were measured in 60 prevalent HD patients with [group I (n=30)] and without [group II (n=30)] HCV, and controls (n=30) by enzyme-linked immunosorbent assay. Parameters related to anaemia, iron metabolism, inflammation, sHJV and hepcidin were measured. Serum hepcidin in HCV positive versus negative groups was 89.40±46.08ng/mL and 224.1±72.36ng/mL, P=0.000, respectively, while sHJV was 245±1.338ng/mL and 254±0.762ng/mL, P=0.147, respectively in positive versus negative patients. In group I, hepcidin correlated with serum ferritin (r=-0.512 P=0.005) and transferrin saturation (TSAT%) (r=0.572, P=0.000) and sHJV correlated with ferritin (r=0.40, P 0.000), TSAT% (r=0.450, P=0.002) and a significant correlation also existed between sHJV and hepcidin (r=-0.259, P=0.045). In the regression analysis, ferritin and TSAT% were able to predict sHJV; (standardized β=0.52, P 0.001) and (standardized β=0.48, P 0.010). Ferritin and sHJV were also able to predict hepcidin (standardized β=0.627, P=0.006) and (standardized β=0.300, P=0.007) in group I. Soluble haemojuvelin levels seem to be associated with iron overload parameters and hepcidin levels in HCV positive HD patients.