Abstract
Serum of freshly killed rats of various ages was treated with <i>p</i>-nitrophenyl phosphate in citric acid buffer solution pH 4.8. Total serum acid phosphatase was determined colorimetrically on the basis of <i>p</i>-nitrophenol formation. Residual serum acid phosphatase was determined on duplicate serum specimens similarly, but with the addition of L (+) tartrate. Prostatic serum acid phosphatase activity was computed by subtraction. This activity was found to increase markedly until prostate weight reached approximately 200mg. after which there was little change in activity. Increases in prostatic weight continued as body weight and age increased. Hence, in young animals prostatic serum acid phosphatase activity is indicative of normal prostatic growth and not necessarily indicative of abnormality.
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