Relationship of Polymorphisms of the Mutation (225131) of the pou1f1 Gene with some Productive Traits in Iraqi Camel Females (Camelus bactrianus).

Abstract

Camels are semi-ruminant placental mammals, classified as two-toed, padded-footed mammals, and belong to the family Camelidae, which includes the one-humped camels (Camelus dromedaries), and the two-humped camels (Camelus bactrianus), llama, Alpaca, Vicuna, and Guanaco. The study used 50 Iraqi single-humped camel females who belonged to private fields in the AL-Furat AL-Awsat region, which involved three Iraqi cities (Babylon, Diwaniyah, and Muthanna). All the Biotechnological and Molecular Genetics analyses were performed in the Altakadum Laboratory, Baghdad, in order to determine the genotypes and their distribution ratios for the POU1F1 gene and the relationship of the Polymorphism of the gene with some productive traits, growth characteristics (weight and body dimensions) and blood biochemical parameters of animals. The mutation 225131 was in the second exon region, in which there was a change in the amino acid C.49 CAA>CAC Gln>His. Three genotypes were discovered in this mutation in the second studied piece, which included the region of the first intron and the second exon, with a length of 777 base pairs using DNA sequencing technology. The results indicated that there were highly significant differences (P≤0.01) in the distribution ratios of the genotypes resulting from the mutation. The results also showed a significant relationship between these genotypes with somebody dimensions, as there was a significant superiority (P≤0.05) for individuals with mutant CC genotype over the wild AA and hetero AC genotypes in each of the traits, body height from the front (220.66±1.76, 215.12±0.92, 212.80 ±2.33) cm and body length (186.66±1.20, 179.47±1.10, 170.00±4.96) cm and head length (55.00±2.08, 50.78±0.46, 51.20 ±1.31) cm for the mutant, wild and hetero, genotypes respectively. Concerning the characteristics of the length of the milking season, daily and total milk production, and its chemical components, there was no significant relationship between the genotypes resulting from the studied mutation.