Abstract
Despite available information on the roles of osmotic pressure, potassium (K+), and calcium (Ca2+) in activation of Eurasian burbot spermatozoon motility, the changes in milt biochemical composition and mechanisms underlying their activation at temperatures above optimal spawning temperature is still unclear. We explored spermatozoon swelling, lipid composition and proteome in relation to osmolality and temperature of swimming medium. The result revealed that temperature increment from 4 to 30°C increases burbot spermatozoa vulnerability to osmotic pressure, decreasing motility in extremely hypotonic media, and the presence of Ca2+decreases swelling of spermatozoa. No spermatozoon swelling was observed in non-ionic isotonic media at all studied temperatures. A role of swelling in activation of spermatozoa motility at 30°C was rejected. No differences were found in protein profile and lipid composition with respect to activation of burbot spermatozoa at 30°C. Burbot spermatozoon activation occurs at 30°C without modification of the spermatozoon membrane. Elucidation of the intrinsic signaling pathway of burbot spermatozoon spontaneous activation requires further study.
Highlights
The Eurasian burbot Lota lota (L.) is the only gadiform fish inhabiting fresh water (Scott and Crossman, 1973) and spawning in midwinter in a light-limited under-ice environment (McPhail, 1997)
We identified two principal components (PC) with PC1 and PC2 describing 44 and 16% of data variability
General Trends Motility duration, velocity, and fertilizing ability of fish spermatozoa may be strongly influenced by ambient temperature (Cosson et al, 1985; Billard et al, 1995; Bombardelli et al, 2013; Dadras et al, 2016) and osmolality (Billard et al, 1995; Wilson-Leedy et al, 2009)
Summary
The Eurasian burbot Lota lota (L.) is the only gadiform fish inhabiting fresh water (Scott and Crossman, 1973) and spawning in midwinter in a light-limited under-ice environment (McPhail, 1997). Restoration program and production of fry has been complemented (Babiak et al, 1998; Hardy et al, 2008). In this context, several studies have been conducted on different aspects of burbot reproduction (Kucharczyk et al, 1998; Lahnsteiner et al, 2004) and fry rearing (Wolnicki et al, 2002; Binner et al, 2008; Zarski et al, 2009). The large interest in restocking and market-sized production of burbot has led to controlled
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