Relationship of follicle stimulating hormone receptor Gene Thr307Ala polymorphism with polycystic ovary syndrome in Iraqi women

Abstract

Polycystic ovary syndrome (PCOS) is a polygenic multifactorial status affecting millions of females worldwide. It is a common cause of anovulatory subfertility. Because of follicle stimulating hormone (FSH), is an important agent in human reproduction. Therefore, the correlation between follicle stimulating hormone receptor (FSHR) gene polymorphisms and polycystic ovary syndrome attracts broad attention. The objective of this study is to investigate the potential association between the follicle stimulating hormone receptor gene Thr307Ala polymorphism with polycystic ovary syndrome in the Iraqi women. A case-control study including 135 Iraqi women of Arab ethnicity (75 PCOS patients and 60 age-matched control women). The age of subjects ranged from 18 to 38 years. PCOS diagnosis was established by Rotterdam consensus criteria. The FSHR (Thr307Ala) variant was tested by conventional polymerase chain reaction (PCR)–restriction fragment length polymorphism (RFLP) followed by deoxyribonucleic acid (DNA) sequencing. The heterozygote Thr/Ala (AG) genotype of Thr307Ala (rs6165) polymorphism of follicle stimulating hormone receptor gene was giving a significant risk (odds ratio=19.4, 95%CI=1.14-30.40, P value=0.002) of developing PCOS in Iraqi women compared with control group. Sequencing analysis of DNA confirms RFLP analysis. In conclusion; the variant Thr307Ala (rs6165) of the follicle stimulating hormone receptor gene is associated with polycystic ovary syndrome and may consider as the causal factor of polycystic ovary syndrome in Iraqi women.