Relationship of Doppler velocimetry parameters with antral follicular population and oocyte quality in Canindé goats

Abstract

The aim of this study was to evaluate the effect of ovarian stimulation protocols on Doppler velocimetry parameters of the ovarian artery and its relationship with the follicular population and oocyte quality. Canindé goats (n=10) were used for ovarian stimulation according to two different protocols: multiple doses (FSH) or a single dose (eCG). The follicular population and ovarian blood flow were determined in the ovarian arteries immediately after treatment through Doppler ultrasonography. To observe the oocyte quality, LOPU was used to aspirate the oocyte from the Graafian follicles and graded. In addition, blood samples were collected for Estradiol concentration. The results showed that the mean number of follicles per animal was higher (P<0.05) in goats treated with multiple doses. However, the resistance index, peak systolic velocity, and pressure gradient of the ovarian artery were similar (P>0.05) between treatment groups. A positive correlation (P<0.05) was observed between the pressure gradient of the ovarian artery and intermediate, large, and total follicles number. A higher (P<0.05) percentage of viable oocytes (GI and GII) were obtained in the multiple dose treatment. A positive (P<0.05) association between oocytes (GII), and peak systolic velocity and pressure gradient were observed. In conclusion, the multiple doses treatment greatly enhances the recruitment of follicles and maintains the oocyte quality. In addition, the use of Doppler ultrasonography positively reveals that the peak systolic velocity, resistance index, and pressure gradient have a positive association with follicular population and oocyte quality.