Relationship of Annona Species as Revealed by PCR-RFLP Analysis.

Abstract

PCR-RFLP of 14 accessions of six Annona species namely A. cherimola Mill., A. squamosa L., A. reticulata L., A. glabra L., A. muricata L. and A. moutana Macfad. and A. cherimola× A. squamosa were studied. One set of Sequence Tagged Site (STS) primers was used in order to amplify the position of cpDNA fragment:‘rbc L-ORFI06'. The amplified fragment was digested with six kinds of restriction enzymes (four base cutter). Enzymes average generated two to eight bands in each sample. Seven different restriction patterns were observed when digested with Rsa I, but other enzymes showed four to six. The pair-wise distance values ranged from 0.017 to 0.607 with the minimum between A. squamosa and A. cher-imola × A. squamosa and the maximum between A. glabra and A. cherimola × A. squamosa. Eight most parsimonious trees were obtained using Swofford parsimony method, and 50 % majority-rule consensus tree was obtained from them. These results indicated that the cultivated and wild species grouped separately and the A. cherimola × A. squamosa was placed between its parents in the cultivated species. A. glabra clustered singly in the dendrogram demonstrating that this species is different from the others. We believe that these results would give basic information in the phylogenetic studies of Annona species using a large number of species.