Relationship etween electroacupuncture and spleen function on leukocyte

Abstract

观察电针治疗白细胞减少症的疗效、 特点及其与脾脏超微结构改变的关系响 探讨针灸调节白细胞的作用机制。 通过家兔静脉注射环磷 酰胺复制白细胞减少症 模型响采用电针治疗响分别每天于耳缘静脉采血进行外周血白细胞计数和分类响观察血 象变化响并于处死前麻醉动物响开腹取脾组织电镜下观测脾血窦基膜小孔口径大小。 与模型对照组比较响电针治疗组动物外周血白细胞数量迅速上升响粒细胞发生核右 移 (P<0.01)。 电针治疗组脾血窦基膜小孔口径大于模型对照组(P<0.05)。 电针可 以使白细胞减少的机体白细胞数量迅速得到恢复响在治疗的早期主要与调节脾脏功能有 关。 To explore the underlying mechanism by which acupuncture regulates the peripheral leukocyte count and observe the relationship between the effects of electroacupuncture (EA) and the spleen ultrastructure in leukopenia. Leukopenia models of rabbits were established by injecting cyclophosphamide (CY) into rabbits’ ear vein, and then the rabbits were treated with acupuncture. The peripheral leukocyte count and classification were measured daily. At last, after the animals were anesthesized, the abdominal cavity was opened, and a piece of spleen tissue was cut. The diameter of splenic sinusoid basal lamina eyehole was measured under electric microscope. The peripheral blood leukocyte count in the EA group increased significantly with shift to right of the granulocyte nuclei compared with model control group (P<0.01). Moreover, the calibers of splenic sinusoid basal lamina eyehole in the EA group were larger than those in the model control group (P<0.05). Acupuncture can enhance the peripheral leukocyte count by promoting spleen activity in the early phase of leukopenia.