Relationship between tree nitrogen status, xylem and phloem sap amino acid concentrations, and apparent soil nitrogen uptake by almond trees (Prunus dulcis)

Abstract

SummaryFour N application rates generated a range of tree N status as indicated by leaf N concentrations in six year old trees of `Mission' almond (Prunus dulcis (Mill.) D. A. Webb). Leaf N concentrations were related positively with concentrations of amino acids - especially asparagine and glutamine - in phloem and xylem saps. A pulse of 1.25 kg N per tree was applied to trees previously receiving 0, 78, 157 or 313 g N per tree, annually. Apparent uptake of soil N into above-ground tree parts was determined over a 39 d period. N uptake was determined as the difference in the products of tree biomass and tissue N concentrations before and after the N pulse. Tissue N concentrations increased signi®cantly in trees previously receiving the two lowest N application rates, whereas trees previously receiving the high N treatments were unresponsive. Tree N contents (0 g N per year, and also 78 g N per year treatments) increased slightly in response to the N pulse, although statistical differences were not observed due possibly to high variability in biomass among trees. At the same time, the amino acid concentrations in phloem exudate and xylem sap were highest in trees grown under the highest N fertilization regime. We suggest that the high amino acid concentrations in the phloem and xylem saps are indicative of a larger pool of amino N cycling throughout the vasculature of high N-status trees. Our data indicate an inverse relationship between tree N status, amino acid concentrations in xylem and phloem saps and capacity for soil N uptake in ®eld-grown trees. These results represent the ®rst ®eld data to correlate the concentration of amino acids circulating in the plant vasculature with soil N uptake. A functional relationship between the amino acid concentrations cycling within the tree and the regulation of soil N uptake has, however, not yet been established.