Relationship between the polymorphisms of B7-H4 gene and the prognosis of breast infiltrative ductal carcinoma in Chinese Han females

Abstract

Objective To investigate the association between the polymorphisms of B7-H4 gene (in cluding rsl0754339, rs10801935 and rs3738414) and the prognosis of infiltrating ductal carcinoma in Chinese women of Heilongjiang province. Methods Two hundreds and eighty Chinese females with infiltrative ductal carcinoma were included in the study. The single nucleotide polymorphisms (SNPs) of rsl0754339, rsl0801935 and rs3738414 in B7-H4 gene were genotyped by PCR-RFLP. The association of the SNPs of B7 H4 gene with the clinical features of breast infiltrating ductal carcinoma (including tumor size, lymph node in volvement , and the expression status of ER, PR and P53) were analyzed through statistical software (the P val ue was calculated using chi-square test) , and the relevance between B7-H4 gene and the prognosis of breast cancer in Chinese Han women of Heilongjiang province was further determined. Results In rs10754339, the frequencies of AA and AG genotypes were significantly different between the ER positive and negative patients (χ~2 =4.06, P<0.05;χ~2 =3.97, P<0.05, respectively) ; the frequencies of AA genotype and G allele were quite different in patients with different PR status (χ~2 =4.74, P<0.05;χ~2=5.30, P<0.05, respectively). In rs10801935, there were significantly differences of the AA genotype and C allele frequencies in patients with different PR status ( χ~2 =5.36, P< 0.05; χ`2 = 5.90, P<0.05, respectively), while the frequency of AA gen otype was significantly different in patients with different P53 status ( χ~2 = 5.82, P <0.05). Conclusion The polymorphisms of B7-H4 gene appear to be associated with the expression of ER, PR and P53 gene in the breast infiltrative ductal carcinoma patients, which suggests that there may be some relationship between the polymorphisms of BT-H4 gene and the prognosis of breast eancer in the population of Heilongjiang province, China. Key words: B7-H4; Infiltrative ductal carcinoma; Single nucleotide polymorphism