Abstract
Prolactin (PRL) plays an important role in promoting mammalian mammary gland development, and milk production during lactation. Therefore the PRL gene was chosen as a candidate gene for milk traits in Holstein dairy cows. PCR-SSCP and PCR- RFLP were used to analyze genetic variations in the 5' regulation region of the PRL gene. In this part of the gene, two new polymorphic sites were detected in the Chinese Holstein dairy cows. One was a XbaI-RFLP locus, and the other was an SSCP locus. Statistical analysis showed that the XbaI-RFLP locus and the SSCP locus had a significant positive effect on milk traits. (Asian-Aust. J. Anim. Sci. 2006. Vol 19, No. 4 : 459-462)
Highlights
MATERIALS AND METHODSProlactin (PRL), one member of growth hormone/prolactin family, is a polypeptide hormone that is synthesized in and secreted from specialized cells of the anterior pituitary gland, the lactotrophs, in vertebrate
SSCP analysis The PCR products were separated on 8% denaturing polyacrylamide gel
Two positive clones of each genotype were sequenced by automatic PE377 sequencer
Summary
MATERIALS AND METHODSProlactin (PRL), one member of growth hormone/prolactin family, is a polypeptide hormone that is synthesized in and secreted from specialized cells of the anterior pituitary gland, the lactotrophs, in vertebrate. The PRL gene was chosen as a candidate gene for milk traits in Holstein dairy cows. PCR-SSCP and PCRRFLP were used to analyze genetic variations in the 5’ regulation region of the PRL gene.
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