Relationship between the plasma expression of microRNA-145 and formation of coronary collateral circulation in patients with coronary artery disease

Abstract

Objective To investigate the relationship between the expression of microRNA-145 (miRNA-145) in plasma and the formation of coronary collateral circulation (CCC) in coronary artery disease patients. Methods A total of 80 patients with coronary stenosis were recruited. The CCC degree was rated by Rentrop rating system: rate 0 (n=24) , rate 1 (n=19) , rate 2 (n=20) , rate 3 (n=17). Plasma expression of miRNA-145 and concentration of VEGF were measured by real-time PCR and ELISA kit respectively. The severity of coronary artery atherosclerosis was evaluated by Gensini score system. Results The concentrations of VEGF in patients with coronary artery disease were increased according to CCC rating, whereas the expressions of miRNA-145 were decreased according to CCC rating. Correlation analysis demonstrated that there was a positive correlation between the plasma concentration of VEGF and CCC rating (r=0.672, P<0.01); while the expression of miRNA-145 in plasma showed a significantly negative correlation with the CCC rating and concentration of VEGF in those patients respectively (r=0.761, P<0.01, r=-0.529, P<0.01). Conclusions In patients of coronary artery disease with better CCC formation, the expression of miRNA-145 in plasma is significantly lower than those with poor CCC formation, these findings may indicate that miRNA-145 may play an important role in the formation of CCC. Key words: Atherosclerosis; Angioplasty, transluminal, percutaneous coronary; Collateral circulation; MiRNA-145