Relationship between the expression of tumor necrosis factor-α and the content of serum tmnor necrosis factor-α in perihematomal tissue and apoptosis in patients with hypertemive intracerebral hemorrhage

Abstract

Objective To investigate the relationship between the expression of tumor necrosis factor-α （TNF-α） and the content of serum TNF-α in perihematomal tissue and apoptosis in patients with hypertensive intracerebral hemorrhage. Methods Ihirty patients with hypertensive intracerebral hemorrhage were randomly divided into 〈6 h, 6-12 h, 12-24 h, 24- 48 h, 48-72 h and 〉 72 h groups according to the time from symptom onset to surgery （n = 5 in each group）. 3here were no significant differences among the basic clinical situation, amount of bleeding, bleeding sites, preoperative Glasgow coma scale scores and operation duration in all groups. A double-antibody sandwich enzyme-linked immunosorbent assay was used to determine the content of serum TNF-α in perihematomal tissue. Immunohistochemistry was used to detect the expression of TNF-a in perihematomal tissue, and TUNEL staining was used to detect the apoptotic cells. Results The numbers of TNF-α positive cells and the content of serum TNF-α in perihematomal tissue reached the peaks at 12-24 h and 24-48 h respectively after intracerebral hemorrhage. There was a positive correlation between them （r =0. 924, P 〈0.05）. The numbers of TNF-α positive cells in perihematomal tissue and the numbers of apoptotic cells were positively correlated （r = 0. 871, P 〈 0. 05）. Conclusions The content of serum TNF-α, the numbers of TNF-α positive cells and apoptotic cells in perihematomal tissue were increased significantly. The numbers of TNF-α positive cells in perihematomal tissue were positive correlated with the content of serum TNF-α and the numbers of apoptotic cells in perihematomal tissue. Key words: Cerebral hemorrhage; Hypertension; Tumor necrosis factor α; Apoptosis