Relationship between the expression of Geminin, CDK4 and biological behaviors of colorectal carcinoma

Abstract

Objective To study the relationship between the expression of Geminin and cyclin dependent kinase 4 (CDK4) and the biological behavior and prognosis of colorectal carcinoma. Methods 50 cases specimen of colorectal carcinoma and complete follow-up data were collected from May 2011 to May 2014. SP immunochemical method was performed to detect the expression of Geminin and CDK4 in 50 cases of colorectal carcinoma, 20 cases of colorectal adenomas and 20 cases of normal colorectal tissues. SPSS 13.0 software was used for statistical analysis. Chi square test and Spearman correlation analysis were used to analyze the data. P<0.05 was statistically significant for the difference. Results The positive rates of Geminin and CDK4 in colorectal carcinoma tissues, colorectal adenomas and normal colorectal mucosa tissues group were gradually decreased, and there were significant difference between each group (P<0.05). The expressions of Geminin and CDK4 were closely related to the degree of differentiation, lymph node metastasis, Duke’s stages and the deepness of invasion (P<0.05). The expression of Geminin was positively correlated with CDK4 (P<0.05). The 3 years survival rates after operation in positive expression groups of Geminin (23.8%) and CDK4 (22.5%) were significantly lower than those in negative expression groups (60.0%). Conclusion The abnormal expressions of Geminin and CDK4 may participate in the occurrence and development of colorectal carcinorma. The detection of both may be used as an important reference index for clinical judgement of malignancy, metastasis and prognosis of colorectal carcinoma. Key words: Colorectal Neoplasms; Geminin; Cyclin-Dependent Kinase 4; Immunohistochemistry