Relationship between TGF-Beta 1 rs1800469 Gene Polymorphism with Degree of Tumor Invasion of Colorectal Cancer at RSUP Prof. Dr. I.G.N.G. Ngoerah Denpasar Bali

Abstract

The incidence of cancer, including colorectal cancer (CRC), increases with age. The Transforming Growth Factor-Beta 1 (TGF-B1) rs1800469 gene polymorphism is associated with the pathogenesis of CRC. The T allele is associated with increased transcriptional activity compared to the C allele, meaning that individuals with TT homozygotes will produce higher TGF-B1 protein concentrations. This study aimed to analyze the relationship between the TGF-B1 rs1800469 gene polymorphism and degree of tumor invasion of CRC in Bali, especially at the RSUP Prof. Dr. I.G.N.G. Ngoerah Denpasar. This hospital study was a cross-sectional study using formalin-fixed-paraffin embedded (FFPE) samples from CRC patients, stored in the Department of Pathology Prof. Dr. I.G.N.G. Ngoerah Denpasar Bali. Identification of TGF-B1 rs1800469 polymorphism gene was performed using polymerase chain reaction (PCR) and sequencing methods. Data on age, sex, histopathological grading, degree of tumor invasion, pathological staging N and tumor location were recorded from the patient's medical record, carried out descriptive analysis and hypothesis testing of bivariate Chi Square analysis. Out of 48 samples, majority was over 50 years old (83.3%), male (54.2%), low grade histopathology (93.8%), adenocarcinoma histological classification (97.9%), high degree of tumor invasion (81.2%), staging pathology N with regional lymph nodes cannot be assessed (93.7%), left tumor location (58.3%) with TGF-B1 rs1800469 gene polymorphism CT genotype of 23 samples (47.9%), followed by TT genotypes of 17 samples (35.4%) and CC genotypes of 8 samples (16.7%). The results of the Chi Square statistical analysis test found no association between the TGF-B1 rs1800469 gene polymorphism in any genotype and degree of tumor invasion of CRC (p=0.688). It can be concluded that there was no significant relationship between the TGF-B1 rs1800469 gene polymorphism and degree of tumor invasion of CRC patients at RSUP Prof. Dr. I.G.N.G. Ngoerah, Denpasar, Bali. Keywords: Colorectal Cancer, Gene Polymorphism, SNP, Tumor Invasion