Relationship between survivin/HLA classI molecules expression and survival of patients withc lear cell er nal cell carcinoma

Abstract

Objective To investigate the expression of survivin/Human leukocyte antigen class I （ HLA-Ⅰ） proteins and its physiological significance in clear cell renal cell carcinoma （ CCRCC ） . Methods Immunohistochemistry was used to analyze survivin/HLA-Ⅰ protein expression in 90 cases of CCRCC and 10 normal tissues to study relationships with clinical symptoms and disease prognosis . Resutl s The level of survivin protein expression was found to be significantly higher in CCRCC tissues 82.2%（ 74/90） than in normal tissues（ 0/10）.However, the relative amount of HLA-Ⅰprotein in colorectal cancer tis-sue was also found to be significantly lower 67.8%（61/90） than in normal tissues 90%（9/10）.Survivin expression was associated with tumor grade , stage,and lymph node metastasis （ P=0.000, P=0.016, and P=0.001, respectively ） .Conversely , lost HLA-Ⅰexpression did not have any associations with clinicopath-ological data （P〈0.05）.Survivin negative patients （25.0%, 4/16） had a higher tumor-free survival rate than patients （52.7%, 39/74）with survivin expression （P=0.037）.Patients （27.6%, 8/29） with normal HLA-Ⅰlevels had a higher tumor-free survival rate than those （ 60.7%, 37/61） with reduced HLA-Ⅰlev-els （P=0.020）.The univariate and multivariate analyses indicated that expression of survivin and HLA indi -vidually and in combination were independent predictors for CCRCC patient survival . Conclusions Over-expression of survivin but reduced HLA-Ⅰ expression is associated with CCRCC development and progres-sion. Key words: Clear cell renal cell carcinoma; Survivin; HLA class I molecules; Immunohisto-chemistry; Prognosis