Relationship between some morphological traits of the tomato lines and resistance to tomato yellow leaf curl virus disease

Sinan Zengin,Aylin Kabas,Hulya Ilbi

doi:10.15835/nbha48111732

Sinan Zengin, Aylin Kabas + Show 1 more

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https://doi.org/10.15835/nbha48111732

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Abstract

Tomato (Solanum lycopersicum L.) is the most produced and exported vegetable in Turkey. There are many pathogens to limit tomato production by reducing yield and fruit quality. Among them, Tomato Yellow Leaf Curl Virus (TYLCV) causes important economic losses. The most efficient and environmentally friendly method against TYLCV is the use of resistant varieties. In this trial, it was aimed to determine some morphological traits which were linked to Ty-1 and Ty-3a genes which confer to TYLCV. A commercial hybrid carrying Ty-1, Ty-3a genes as heterozygous was crossed to a susceptible inbred line from Bati Akdeniz Agricultural and Research Institute (BATEM). Marker assisted selection (MAS) was carried out in F1 and F2 generations and biological tests were done for TYLCV resistance in F3 generation. MAS for Ty-3a and Ty-1 genes were compatible with biological tests. In total of 95 genotypes in F3 were developed with molecular marker selection. It was determined that 30 genotypes having the Ty-3a and Ty-1 genes as homozygous resistant. The nine genotypes carried these genes in heterozygous form. 56 genotypes were identified as susceptible. The 43 morphological traits were observed in identified individuals to correlate with resistant allele, Ty-3a. It was found that there was statistically important correlation between Ty-3a and length of internode, length of stem at first inflorescence, status of calix, leaf attitude, length of inflorescence and plant habitus. Also, there was negative correlation between fruit weight, fruit length and resistance. Therefore, we identified some morphological markers linked to Ty-3a which can be used in selection for TLYCV resistant breeding programme.

Highlights

Tomato (Solanum lycopersicum L.,) is one of the most economically important vegetables in the world
Segregation of Ty-1 and Ty-3a by molecular markers F1 plants which were obtained by crossing Tayfun F1 and BATEM1 pure line, were screened with molecular markers linked to Ty-1 and Ty-3a
Individuals of F2 and F3 population were screened via molecular markers to determine segregation of the Ty-1 and Ty-3a genes

Summary

Introduction

Tomato (Solanum lycopersicum L.,) is one of the most economically important vegetables in the world. The world tomato production is over 163 million tons from an area of 4.83 million hectares (FAO, 2017). Fruit quality, shelf-life, and nutritional content are reduced when tomato crops can be infected by diseasecausing bacterial, fungal, and viral pathogens (Oerke, 2006; Hanson et al, 2016). The tomato yellow leaf curl virus (TYLCV) is the most destructive disease and causes loss of yield between 85% and 100% (Pico et al, 1996; Czosnek and Laterrot, 1997; Polston and Anderson, 1997). TYLCV is transmitted by Received: 19 Nov 2019. Received in revised form: 18 Mar 2020.

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