Abstract
Background/aim Age-related cataract is the most important visual impairment all over the world. Epigenetic modifications, especially overexpression of histone deacetylases, have become the focus of interest for cataract development in recent years. Sirtuin 1 (SIRT1), a class II histone deacetylase and a member of the sirtuin family, is one of the best-characterized histone deacetylases and has a pivotal role in age-related diseases. However, the association of SIRT1 with age-related cataracts has not yet been fully elucidated. Therefore, we aimed to determine the expression of SIRT1 in age-related cataract patients.Materials and methods Expressions of SIRT1 were evaluated by quantitative polymerase chain reaction (qPCR) in patients and healthy controls. RNA samples were collected from the anterior capsule and peripheral blood samples of age-related cataract patients. Human lens epithelial cell line B3 and peripheral blood samples of healthy subjects were used as controls. Results We determined that the expression of SIRT1 in blood and anterior capsule samples increased significantly compared to the control group (P < 0.05). Conclusion The expression level of SIRT1 plays a vital role in the development of age-related cataract and it can be used as a biomarker. Thus, SIRT1 inhibitors can be used in the treatment of age-related cataract disease.
Highlights
A Cataract is a blurring of the natural lens in the eye
We determined that the expression of Sirtuin 1 (SIRT1) in blood and anterior capsule samples increased significantly compared to the control group (P < 0.05)
The expression level of SIRT1 plays a vital role in the development of age-related cataract and it can be used as a biomarker
Summary
A Cataract is a blurring of the natural lens in the eye. The main cause of cataract is aging, trauma can occur due to toxic causes, systemic disorders (e.g., diabetes), smoking, and hereditary factors [1]. Age-related cataract is the most common cause of vision loss. Studies showed that the incidence of cataract was 50% in the 65–74 age group and more than 70% in those over 75 years [2]. There are many causes of opacification of the lens fibers, the first and most important cause is aging [1]. The development of cataract depends on many factors and it is usually characterized by deterioration of the lens microstructure. Age-related cataracts develop as a result of interaction between genes and environmental factors that affect the phenotype [3]
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