Abstract
Purpose: The aim of this study was to unravel the link between human leukocyte antigen-G untranslated region (HLA-G 3-UTR) single-nucleotide polymorphism (SNP) and preeclampsia by examining polymorphisms in HLA-G 3-UTR in preeclampsia patients and their newborns, as well as those of women with normal pregnancy and their newborns.Methods: Single pregnant mothers and their newborns at the Third Affiliated Hospital of Zhengzhou University, China, were divided into preeclampsia group (144 cases), and normal pregnancy group (122 cases). Blood samples from the two groups were collected for DNA extraction, and the DNA samples were analyzed for HLA-G 3'-UTR SNP by polymerase chain reaction (RT-PCR) and direct sequencing method.Results: The frequency of CG genotype in HLA-G 3-UTR 3127 gene in the preeclampsia group was significantly lower, while the frequency of AA genotype in HLA-G 3-UTR 3172 gene in the preeclampsia group was significantly higher (p < 0.05) when compared with the normal pregnancy group. Furthermore, frequencies of the compatible genotype AA/AA (mothers/newborns) in HLA-G 3-UTR 3172 gene in the preeclampsia group were significantly higher relative to the normal pregnancy group (p< 0.05).Conclusion: These results suggest that when CC/GG is the compatible genotype in HLA-G 3-UTR 3127 gene of mothers/newborns, preeclampsia risk may decrease, but it may increase if the compatible genotype is AA/AA. Thus, SNP in HLA-G 3-UTR 3127 gene may be a protective factor against preeclampsia in Chinese Han pregnant women, while SNP in 3172 gene may be associated with susceptibility to preeclampsia.Keywords: Single-nucleotide polymorphisms, Human leukocyte antigen-G, Un-translated Region, Preeclampsia
Highlights
Preeclampsia is a hypertensive disorder in pregnancy, a multiple organ dysfunction of pregnant women during the second and third trimesters of pregnancy
Results of analysis of single-nucleotide polymorphism (SNP) genotype frequency distributions of Human leukocyte antigen (HLA-G) 3-untranslated region (3-UTR) in pregnant women and embryos from the preeclampsia and normal pregnancy groups satisfied the law of HardyWeinberg equilibrium (p > 0.05)
The study showed that in HLA-G 3-UTR, three single-nucleotide polymorphisms in HLA-G 3-UTR occurred at site 3127; site 3172 site, and site 3181 site
Summary
Preeclampsia is a hypertensive disorder in pregnancy, a multiple organ dysfunction of pregnant women during the second and third trimesters of pregnancy. It is characterized by hypertension, proteinuria and edema 20 weeks into pregnancy. Other researchers are of the view that immune tolerance can protect the embryo as a homologous graft from rejection by the maternal immune system [3,8]. Trophoblast cells may invade the myometrium and spiral artery without being identified and excluded by the maternal immune system. This is associated with MHC expressed in trophoblast cells. HLA-G occupies a crucial place in maternal-fetal immune tolerance and the rebuilding of placental vessels [5,9,10]
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