Abstract
The relationship between bone marrow mesenchymal stem cells (BMSCs) and aging, as well as the antiaging effects of BMSCs, was observed. An aging macaque BMSC model was established. We isolated BMSCs from young and aged macaques and used RT-PCR and Western blot to confirm the aging-related mRNAs and their expression, revealing that TERT, SIRT1 and SIRT6 expression was decreased in the aged BMSCs. The morphology, immunophenotype, differentiation potential, proliferation potential, and antiaging effects of aged and young BMSCs on 293T cells were compared. The expression of aging-related genes and the difference between the secreted cytokines in natural aging and induced aging BMSCs were observed. The transcriptome of peripheral blood mononuclear cells from macaques was analyzed by high-throughput sequencing. Finally, the transcriptional characteristics and regulatory mechanisms of gene transcription in aging macaques were investigated.
Highlights
Over the 50 years, the aging population in developing countries is expected to grow four-fold
Flow cytometry was performed on P3 bone marrow mesenchymal stem cells (BMSCs), and the cell surface antigens CD29, CD105, CD73, CD184, CD90, CD45 and HLA-DR were detected
The results showed that the percentage of positive BMSC staining in the young group was 1.33 ± 1.51%, which was significantly lower than that in the aged group (35.33 ± 3.88%, p
Summary
Over the 50 years, the aging population in developing countries is expected to grow four-fold. Since the end of the 20th century, China has gradually become an aging society. The problems brought on by aging have become more prominent and pose a serious challenge to the development of the entire society. It is urgent to deal with the diseases caused by aging and the aging body. The body is chronically affected by various environmental factors inside and outside the body, causing the structure and function of various tissues and organs to gradually degenerate, eventually developing chronic tissue syndromes or organ dysfunction [1]. The mechanism of aging has not been thoroughly elucidated to date. The rapid development of cell biotherapy has led to new opportunities to elucidate the diseases related to the elderly. The role of cell biotherapy in this regard has attracted the attention of researchers and achieved important progress
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