Relationship between secondary metabolites and insect loads in cabbage with different leaf shapes and positions.

Abstract

Secondary metabolites in plants play a crucial role in defense mechanisms against insects, pests, and pathogens. These metabolites exhibit varying distributions within and among plant parts under different biotic and abiotic conditions. Understanding the intricate relationships between secondary metabolites and insect populations can be helpful for elucidating plant defense mechanisms and enhancing agricultural managing efficiencies. To investigate the influence of the glucosinolate profile in the leaves of three cabbage (Brassica oleracea var.capitata L.) varieties on insect loads. Glucosinolate profiles across different leaf positions (such as bottom, middle, and center) and leaf shapes (such as curly and non-curly leaf) of three cabbage varieties (Xiagan [XGA], Xiaguang [XGU], and Qiangxia [QIX]) were analyzed by using high-performance liquid chromatography-mass spectrometry (LC-MS). The insect loads were recorded by visually inspecting the upper and lower layers of each target leaf. Increasing concentrations of four glucosinolates, namely, glucoiberin, progoitrin, glucoraphanin, and glucobrassicin, were positively related to insect loads. While increasing concentrations of the other four glucosinolates, such as neoglucobrassicin, 4-methoxyglucobrassicin, sinigrin, and gluconapin, were negatively related to insect loads. Furthermore, both glucosinolate synthesis and insect loads were significantly higher in the curly-shaped and middle-position leaves than in the non-curly-shaped and bottom- and central-position leaves across the cabbage varieties. Differences in glucosinolate profiles across leaf positions and shapes strongly influenced the insect loads of the three Brassica varieties. This link may further extend our understanding of the real defense power of a particular variety against herbivore damage.