Relationship between RANTES gene polymorphism and pre -eclampsia

Abstract

Objective To investigate whether the cytokine regulated upon activation normal T cell expressed and secreted (RANTES) genetic polymorphisms affects occurrence of pre-eclampsia (PE) in pregnant women and its possible mechanism. Methods Using techniques of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) , we studied the single nucleotide polymorphism (SNP) of RANTES in 120 pregnant women with PE (PE group) and 200 healthy pregnant women (control group) in our hospital between August 2012 and August 2015. Western blotting were used to detect RANTES level in placenta. Results There was no significant difference in allele distribution frequency of RANTES-403 and RANTES-28 between the PE and control groups (P>0.05). The placenta RANTES gene expression index was higher in the PE group than in the healthy control group (0.736±0.249 vs 0.468±0.15, P<0.05). In the PE group, significantly lowered expression index of RANTES was seen in subjects with genotype RANTES-403A/A compared to those with genotype RANTES-403 G/G (0.429±0.107 vs 0.743±0.132, P< 0.05). Conclusion RANTES expression was enhanced in placenta of patients with PE, suggesting that it may be involved in occurrence of PE. RANTES single nucleotide polymorphism may be associated with the level of RANTES protein production. Key words: Preeclampsia; Chemokines; Single nucleotide polymorphism