Relationship between QTLs for preharvest sprouting and alpha-amylase activity in rye grain

Abstract

Preharvest sprouting (PHS) and high alpha-amylase activity (AA) negatively affect quality of rye grain. The objective of this study was to reveal genetic relationship between PHS and AA by developing a consensus map of QTLs controlling each trait. A method of composite interval mapping (CIM) was used to search for QTLs within the 541 × Ot1-3 and DS2 × RXL10 F2 mapping populations representing wide variation range of both traits. Sixteen QTLs for AA were detected on chromosomes 1R (3), 2R (2), 3R (2), 4R (3), 5R (3), 6R (2) and 7R (1). Their distribution was not random showing a tendency of QTL location in distal regions of chromosomes. Nine QTLs for AA located on chromosome arms 1RS, 2RL, 3RS, 4RL, 5RS, 5RL, 6RS, 6RL and 7RS coincided with QTLs for PHS. Seven QTLs for AA independent from PHS were detected on chromosome arms 1RL (2), 2RS, 3RL, 4RS, 4RL and 5RL. Four QTLs for PHS not associated with those for AA were identified on chromosomes 1RL, 2RL, 5RL and 7RL. Partial overlapping of the genetic systems controlling AA and PHS suggests that alpha-amylase found in sound grain of rye could be produced through at least three independent mechanisms i.e. PHS at its initial stage, late maturity alpha-amylase (LMA) and/or retained pericarp alpha-amylase (RPAA). Six QTLs co-located on both maps were found on chromosome arms 1RS, 2RS, 5RS, 5RL, 6RS and 6RL. Valuable features of line Ot1-3 i.e. resistance to preharvest sprouting and low alpha-amylase production in ripening grain can be attributed to seven major QTLs from chromosomes 1RL, 2RL, 5RL (2), 6RL and 7R (2). This set of QTLs, identified in line Ot1-3, might be useful in breeding sprouting resistant cultivars of rye.