Abstract
Objective To investigate the association between mutation of H-ras,K-ras,N-ras and carcinogenesis of primary liver cancer (PLC).Methods HotStarTaq polymerase chain reaction (PCR) was used to detect the expression of H-ras,K-ras,N-ras gene in 32 PLC patientds and the mutation of H-ras,K-ras,N-ras gene was analyzed by PCR and direct sequencing.Results The expression of H-ras,K-ras,N-ras was detected in 32 cases.Sequencing indicates that H-ras mutation occur in 17 cases,among which 14 cases was verified to be aA/G transition in codon 40 and 3 cases G/A transition in codon 62.K-ras,N-ras had no mutation.H-ras mutation was correlated to tumor metastasis.Conclusion Abnormal expression of H-ras is correlated with PLC and may lead to the the carcinogenesis of PLC. Key words: Primary liver cancer; HotStarTaq polymerase chain reaction; ras; Sequencing
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