Relationship between polymorphisms of IL-13 gene Intron 3+ 1923 and bronchial asthma in children

Abstract

Objective To explore the correlation of the polymorphism of gene IL-13 Intron 3+ 1923and IL-13 and total IgE with pediatric asthma. Methods Polymerase chain reaction-restriction fragment length polymorphisms was used to analyze the polymorphism of gene IL-13 Intron 3+ 1923 in 50 normal children (healthy control group) and 77 asthma children (asthma group); enzyme linked immunosorbent assay was used to measure serum IL-13 and total IgE, and used the SPSS 18.0 software to analyze the serum IL-13 and total IgE in 2 groups. Results 1.The gene type and allele gene frequency distribution of gene IL-13 site Intron 3+ 1923 in asthma group and healthy control group had statistical significance(genotype: χ2=7.239, P<0.05; allele frequency: χ2=6.731, P<0.01), Allele T had a relationship with asthma.(2)The serum IgE concentration in asthma group was (50.98±27.97) mg/L, and in normal control group was (42.85±27.19) mg/L, contrast difference had statistical significant (t=3.067, P<0.01). (3) The serum IgE in asthma group was (197.13±94.7) ng/L and in healthy control group was (159.06±107.65) ng/L, contrast difference had statistical significant (t=2.079, P<0.05). (4) Different genotype of gene IL-13 Intron 3+ 1923 in asthma group had distinguishing level of IgE: CC was (30.25±19.75) mg/L, CT was (57.50±27.64) mg/L, TT was (65.09±21.14) mg/L, group contrast had significant difference (P<0.05); Different genotype of gene IL-13 Intron 3+ 1923 in asthma group had distinguishing level of IL-13, CC: (105.59±57.32) ng/L, CT: (222.45±74.00) ng/L, TT: (274.38±86.12) ng/L, group contrast had significant difference (P<0.05). Conclusions Polymorphism of gene IL-13 Intron 3+ 1923 is relevant with children asthma, allele T gene is the correlative gene of children asthma.Allele IL-13 Intron 3+ 1923T has an influence on the level of IL-13, IgE, which makes them higher.Increasing total IL-13 and IgE in serum has a relationship with children asthma. Key words: Bronchial asthma; Gene polymorphism; Interleukin-13 Intron 3+ 1923; Interleukin-13; Child