Abstract
BackgroundTo investigate the relationship between polymorphism of scavenger receptor class B member 2 (SCARB2) gene and clinical severity of enterovirus (EV)-71 associated hand-foot-mouth disease (HFMD).MethodsAmong the 100 recruited cases, 56 were in the severe HFMD group (case group) and 44 were in the general HFMD group (control group). By screening functional single nucleotide polymorphisms (SNPs) and hot SNPs, and performing SNP site optimization, some SNP sites of SCARB2 gene were selected for analysis. Genotyping was performed using a MassArray platform. PLINK software was used for statistical processing and analysis of the correlation differences between the mutant genotypes in the severe and general HFMD groups. The relationship between the SNPs and clinical severity of enterovirus (EV)-71 associated HFMD was assessed.Results28 SNPs in SCARB2 were selected by site optimization. Then three loci were not in agreement with the minor allele frequency (MAF) in the 1000 Han Chinese in Beijing (CHB) dataset. Another three loci could not be detected. Nine loci were not suitable for further analysis (MAF < 0.01 and Hardy–Weinberg [HWE] P < 0.001). A total of 13 sites were subsequently analyzed. Through Fisher analysis, the frequency of the rs6812193 T allele was 0.134 and 0.034 in the severe and general HFMD groups, respectively (P 0.023 < 0.05, odds ratio [OR] 4.381 > 1). Logistic regression analysis of rs6812193 T alleles between the severe and general HFMD groups, respectively (P 0.023 < 0.05, OR 4.412 > 1, L95 1.210 > 1). Genotype logistic regression analysis of the rs6812193 alleles CT + TT versus CC gave an OR of 4.56 (95% confidence interval [95% CI] 1.22–17.04, P = 0.012).ConclusionThe rs6812193 T allele was a susceptibility SNP for SHFMD, and the rs6812193 polymorphism might be significantly associated with the susceptibility to EV-71 infection.
Highlights
Hand, foot, and mouth disease (HFMD) is an infectious disease caused by a variety of enteroviruses which belongs to the small ribonucleic acid (RNA) virus family [1]
Yamayoshi et al [12, 13] found that the tissue distribution of EV-71 virus antigen was well correlated with scavenger receptor class B member 2 (SCARB2), and further found that this receptor was involved in the endocytosis and membrane transport of pathogenic bacteria
By using Fisher analysis and allele logistic regression analysis, the rs6812193 T allele was shown to have a pathogenic effect. rs6812193 genotype logistic regression analysis in a dominant model showed that CT + TT genotype carriers had an increased risk of severe hand-foot-mouth disease (HFMD) compared with CC genotype carriers
Summary
Foot, and mouth disease (HFMD) is an infectious disease caused by a variety of enteroviruses which belongs to the small RNA virus family [1]. HFMD is common in children under 5 years of age, and it is mainly manifested as herpes and maculopapules on the hands, feet, mouth, and other areas. Wang et al Virol J (2021) 18:132 rapidly and develop neurogenic pulmonary edema, circulatory disturbance, and even death at 1–5 days after disease onset [2]. HFMD is a global disease with a variety of causes. The main causes of HFMD are enterovirus (EV)-71 and Coxsackievirus A16 infection; EV-71 is responsible for most of the severe cases and fatal cases [4]. To investigate the relationship between polymorphism of scavenger receptor class B member 2 (SCARB2) gene and clinical severity of enterovirus (EV)-71 associated hand-foot-mouth disease (HFMD)
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