Abstract
The current study was designed to explore the association between the pigments production and biofilm construction in local Pseudomonas aeruginosa isolates. Out of 143 patients suffering from burns, urinary tract infections (UTI), respiratory tract infections and cystic fibrosis obtained from previous study by Mahmood (2015), twenty two isolates (15.38%) were identified from (11) hospitals in Iraq, splitted into three provinces, Baghdad, Al-Anbar and Karbala for the duration of June 2017 to April 2018. Characterization was carried out by using microscopical, morphological and biochemical methods which showed that all these isolates belong to P. aeruginosa. Screening of biofilm production isolates was carried out by using nutrient broth supplemented with glucose (0.25%) production medium which encourage this biofilm production. The percentage of pigmented isolates were collected from a total of 143 samples, 2.8% of the isolates from burns, 2.1% isolates from cystic fibrosis and 0.7% isolates from UTI. Quantitative assays for biofilm formation were conducted using ELIZA technique. The results showed that all (22) isolates produced biofilm except one (B1 isolate). Biofilm quantities were varied from strong to medium production in comparison with control (0.0663). Statistical analysis results using Fischer's Exact test (p<0.05) were non-significant, therefore the pigment production has no association with biofilm formation for all of them.
Highlights
Pseudomonas genus is a Gram negative rod belonging to Pseudomonadaceae family, motile by polar flagella [6].Pseudomonas aeruginosa is an extremely common opportunistic pathogen and it has the ability of producing various bioactive molecules such pigments
The high-density of bacterial assemblages associated with chronic infections, for instance biofilm formation, which favors the development of variants, which accomplishment from the homologous recombination and DNA
The current study designed to estimate the association between pigment and biofilm production from local P. aeruginosa isolates and to achieve such goal the following steps were suggested, isolation and identification of local P. aeruginosa isolates from different pathogenic sources,screening of local isolates capability of pigment and biofilm production from previous study, measurement of quantity of biofilm by using ELIZA technique and estimate the relationship between biofilm and pigment production using chi- square analysis
Summary
Pseudomonas genus is a Gram negative rod belonging to Pseudomonadaceae family, motile by polar flagella [6]. Pseudomonas aeruginosa is an extremely common opportunistic pathogen and it has the ability of producing various bioactive molecules such pigments. It has great genomic content (~6.5 Mbp) for variations in metabolism and adaptation for several environmental roles and mismatch repair system (2, 12,13 and 16). The high-density of bacterial assemblages associated with chronic infections, for instance biofilm formation, which favors the development of variants, which accomplishment from the homologous recombination and DNA mismatch repair system (2, 12, and 15). Instant controller by extra of high-dose for antibiotic required for longterm administration. Though, those radical actions are ineffective and lead to significant morbidity and mortality [7]. The current study designed to estimate the association between pigment and biofilm production from local P. aeruginosa isolates and to achieve such goal the following steps were suggested, isolation and identification of local P. aeruginosa isolates from different pathogenic sources ,screening of local isolates capability of pigment and biofilm production from previous study, measurement of quantity of biofilm by using ELIZA technique and estimate the relationship between biofilm and pigment production using chi- square analysis
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