Relationship between photosynthetic radiation‐use efficiency of barley canopies and the photochemical reflectance index (PRI)

Abstract

Some processes of excess radiation dissipation have been associated with changes in leaf reflectance near 531 nm. We aimed to study the relations between the photochemical reflectance index (PRI) derived from this signal, and photosynthetic radiation‐use efficiency (defined as net CO2 assimilation rate/incident photon flux density) in a cereal canopy. Measurements of reflectance, fluorescence, gas exchange and xanthophyll cycle pigments were made in the morning, midday and afternoon in barley canopies with two levels of nitrogen fertilization. The photosynthetic radiation‐use efficiency decreased at midday, mainly in the third leaf, in both treatments, with lower values for the nitrogen deficient leaves. The zeaxanthin content showed the inverse pattern, increasing at midday and in the nitrogen deficient treatment. The photosynthetic radiation‐use efficiency was well correlated with the epoxidation state, EPS (violaxanthin + 0.5 antheraxanthin)/(violaxanthin + antheraxanthin + zeaxanthin). The PRI [here defined as (R539 ‐ R570)/(R539+ R570)] was significantly correlated with epoxidation state and zeaxanthin and with photosynthetic radiation‐use efficiency. These results validate the utility of PRI in the assessment of radiation‐use efficiency at canopy level.