Abstract
Because of the paucity of data on phospholipid transfer protein (PLTP) activity and lipoprotein phospholipid in mouse strains, plasma PLTP activity (PLTA), plasma phospholipid and cholesterol, HDL phospholipid and cholesterol, and HDL size distribution were determined in 15 inbred mouse strains. The 15 inbred mouse strains differed in their relatedness to one another and consisted of six largely unrelated groups: Castaneus, Swiss, C57BL, AKR, DBA, and NZB. Lipid and PLTA analyses were performed on plasma pools from male and female mice that had fasted for 4 h prior to blood draw. Among the representative unrelated strains fed the chow diet, there was a highly significant relationship between PLTA and plasma phospholipid (rs = 0.727, P < 0.01), HDL phospholipid (rs = 0.762, P < 0.01), HDL cholesterol (rs = 0.699, P < 0.02), percentage of large HDL particles (rs = 0.699, P < 0.02), and HDL peak size (rs = 0.776, P < 0.01). Similar results were obtained among these strains fed a high fat, high cholesterol diet. PLTA increased in all strains fed the high fat diet ( x‒ = 94%, range 6 to 221%). Strain SM having relatively low PLTA and HDL was crossed with strain NZB having high PLTA and HDL. The F1 progeny from this cross were backcrossed to strain SM and 41 male backcross progeny collected. Among these individual backcrossed animals, PLTA was highly correlated with plasma phospholipid (rs = 0.508, P = 0.001), HDL phospholipid (rs = 0.566, P < 0.001), HDL cholesterol (rs = 0.532, P < 0.001), and percentage of large HDL particles (rs = 0.446, P = 0.020). Therefore, we conclude that PLTP is a determinant of HDL level and size in mice.—Albers, J. J., W. Pitman, G. Wolfbauer, M. C. Cheung, H. Kennedy, A-Y. Tu, S. M. Marcovina, and B. Paigen. Relationship between phospholipid transfer protein activity and HDL level and size among inbred mouse strains. J. Lipid Res. 1999. 40: 295–301.
Highlights
Because of the paucity of data on phospholipid transfer protein (PLTP) activity and lipoprotein phospholipid in mouse strains, plasma PLTP activity (PLTA), plasma phospholipid and cholesterol, high density lipoprotein (HDL) phospholipid and cholesterol, and HDL size distribution were determined in 15 inbred mouse strains
54–88% of the total cholesterol was found in the HDL-cholesterol fraction, with the remainder residing in the very low density lipoprotein (VLDL) and LDL fractions
Among these 15 mouse strains there are six, largely unrelated groups: Castaneus, which is recently inbred from wild mice; AKR/J as a representative from Furth’s stocks; DBA and related strains including DBA/2J, A/HeJ, A/J, BALB/ cJ, and C3H/HeSnJ; Swiss mice, which include SWR/J and SJL/J; C57 from Miss Lathrop’s stocks which include C57BL/6J, C57BLKS/J, and C57L/J; and NZB/BINJ because it is unrelated to the other groups
Summary
Because of the paucity of data on phospholipid transfer protein (PLTP) activity and lipoprotein phospholipid in mouse strains, plasma PLTP activity (PLTA), plasma phospholipid and cholesterol, HDL phospholipid and cholesterol, and HDL size distribution were determined in 15 inbred mouse strains. The F1 progeny from this cross were backcrossed to strain SM and 41 male backcross progeny collected Among these individual backcrossed animals, PLTA was highly correlated with plasma phospholipid (rs ؍0.508, P ؍0.001), HDL phospholipid (rs ؍0.566, P Ͻ 0.001), HDL cholesterol (rs ؍ 0.532, P Ͻ 0.001), and percentage of large HDL particles (rs ؍0.446, P ؍0.020). In order to assess potential relationships between PLTA and HDL among mouse strains, we have conducted a comprehensive survey of plasma lipids, PLTP activity, HDL levels, and HDL size in both male and female mice fed a chow diet and a high fat, high cholesterol diet
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