Relationship between phospholipid transfer protein activity and HDL level and size among inbred mouse strains

Abstract

Because of the paucity of data on phospholipid transfer protein (PLTP) activity and lipoprotein phospholipid in mouse strains, plasma PLTP activity (PLTA), plasma phospholipid and cholesterol, HDL phospholipid and cholesterol, and HDL size distribution were determined in 15 inbred mouse strains. The 15 inbred mouse strains differed in their relatedness to one another and consisted of six largely unrelated groups: Castaneus, Swiss, C57BL, AKR, DBA, and NZB. Lipid and PLTA analyses were performed on plasma pools from male and female mice that had fasted for 4 h prior to blood draw. Among the representative unrelated strains fed the chow diet, there was a highly significant relationship between PLTA and plasma phospholipid (rs = 0.727, P < 0.01), HDL phospholipid (rs = 0.762, P < 0.01), HDL cholesterol (rs = 0.699, P < 0.02), percentage of large HDL particles (rs = 0.699, P < 0.02), and HDL peak size (rs = 0.776, P < 0.01). Similar results were obtained among these strains fed a high fat, high cholesterol diet. PLTA increased in all strains fed the high fat diet ( x‒ = 94%, range 6 to 221%). Strain SM having relatively low PLTA and HDL was crossed with strain NZB having high PLTA and HDL. The F1 progeny from this cross were backcrossed to strain SM and 41 male backcross progeny collected. Among these individual backcrossed animals, PLTA was highly correlated with plasma phospholipid (rs = 0.508, P = 0.001), HDL phospholipid (rs = 0.566, P < 0.001), HDL cholesterol (rs = 0.532, P < 0.001), and percentage of large HDL particles (rs = 0.446, P = 0.020). Therefore, we conclude that PLTP is a determinant of HDL level and size in mice.—Albers, J. J., W. Pitman, G. Wolfbauer, M. C. Cheung, H. Kennedy, A-Y. Tu, S. M. Marcovina, and B. Paigen. Relationship between phospholipid transfer protein activity and HDL level and size among inbred mouse strains. J. Lipid Res. 1999. 40: 295–301.