Relationship between Peripheral Plasma Inhibin and Progesterone Concentrations in Sahiwal Cattle (Bos Indicus) and Murrah Buffaloes (Bubalus bubalis)

Abstract

The present study investigated peripheral plasma immunoreactive inhibin (ir-inhibin) concentrations in relation to the stage of oestrous cycle and progesterone concentrations in cycling Sahiwal cattle (Bos indicus) and Murrah buffaloes (Bubalus bubalis). Blood samples were collected once daily for thirty-two consecutive days from cattle and buffaloes (5 each) during winter months of January and February. Mean (±S.E.M.) plasma ir-inhibin concentrations ranged from 0.40±0.01 to 0.59±0.03 ng/ml in cattle and from 0.29±0.03 to 0.52±0.05 ng/ml in buffaloes. In cattle, ir-inhibin concentrations increased from 0.47±0.07 ng/ml on day -4 (day 0=day of oestrus) to reach a maximum value of 0.59±0.03 ng/ml on day -2. Thereafter, ir-inhibin concentrations showed a decline to reach a low of 0.40±0.01 ng/ml on day 11 of the oestrous cycle. In buffaloes, ir-inhibin concentrations increased from 0.38±0.04 ng/ml on day -4 to reach a maximum concentration of 0.52±0.05 ng/ml on day -2. Ir-inhibin concentrations then declined to reach a low of 0.29±0.03 ng/ml on day 9 of the cycle. In both cattle and buffaloes, ir-inhibin concentrations which were lowest (0.43±0.02 and 0.34±0.02 ng/ml, respectively) during the mid-luteal phase of the oestrous cycle increased (p<0.05) to 0.52±0.03 and 0.44±0.04 ng/ml, respectively, during the late luteal phase, and then further to the highest value of 0.53±0.02 and 0.49±0.04 ng/ml, respectively, during the perioestrus phase, following which these declined to 0.50±0.02 and 0.39±0.03 ng/ml, respectively, during the early luteal phase. The variations in peripheral plasma ir-inhibin profile in both the species appear to be related to the changes in characteristics of follicular populations during the oestrous cycle. Peripheral plasma ir-inhibin concentrations were negatively correlated with progesterone concentrations in cattle (r=-0.51, p<0.01) and buffaloes (r=-0.30, p<0.01) indicating that the corpus luteum is not a source of peripheral ir-inhibin in these species. (Asian-Aust. J. Anim. Sci. 2003. Vol 16, No. 1 : 6-10)