Relationship between Parameters Linked to Surgery, Hospitalization and Single Nucleotide Polymorphism in Patients with Colorectal Cancer.

Abstract

Colorectal cancer (CRC) is the third most common type of cancer, the fourth leading cause of cancer related deaths worldwide and a major public health issue. Management is difficult, especially in elderly patients, and efforts for an individualized treatment are an asset for patients' wellbeing. Factors such as cumulated comorbidities, disease stage, and operative time may increase the length of hospitalization (LOH) and overall costs. The aim of this paper is to assess the impact of a single nucleotide polymorphism (SNP) - rs6983267 - on CRC risk in Romanian individuals. A case-control genotyping molecular study was performed on 32 patients diagnosed with CRC (median age 67.5 years) who underwent elective surgery and 30 patients withour CRC (median age 66 years). Genotyping rs6983267 was performed on DNA extracted from peripheral venous blood. Twenty fice patients were diagnosed with colonic cancer with different localizations, whereas seven had rectal cancers. Median LOH was 16.5 days. Genotyping for rs6983267 revealed no heterozygous (G/T) individuals within the control group, with all patients showing homozygous profiles (76.67% G/G, and 23.33% T/T), but the heterozyhous (G/T) genotype was present in 59.38% of the patients in the study group (with 21.88% G/G and 18.75% T/T genotypes). A higher percentage of CRC patients had at least one G allele (81.21%) when compared to controls (76.57%), although G allele frequency was higher in the control group due to an increased percentage of G/G homozygosity. When comparing clinical data between groups, we found an association between the lenght of hospitalization and factors that influenced operating time. Further research is still necessary to accurately calculate a CRC risk associated with the presence of this SNP in a Romanian population.