Relationship between nitrifier and denitrifier community composition and abundance in predicting nitrous oxide emissions from ephemeral wetland soils

Abstract

The link between differences in the community composition of nitrifiers and denitrifiers to differences in the emission of nitrous oxide (N 2O) from soils remains unclear. Nitrifier and denitrifier community composition, abundance and N 2O emission activity were determined for two common landscapes characteristic of the North American “prairie pothole region”: cultivated wetlands (CW) vs. uncultivated wetlands (UW). The hypotheses of this study were: (1) landscape selects for different nitrifier and denitrifier communities, (2) denitrification was the dominant N 2O emitting process, and (3) a relationship exists between nitrifier and denitrifier community composition, their abundance, and N 2O emission. Comparisons were made among soils from three CW and three UW at the St. Denis National Wildlife Area. Denaturing gradient gel electrophoresis was used to compare community composition, and quantitative polymerase chain reaction was used to estimate community size. Incubation experiments on re-packed soil cores with 15N-labeled nitrate were performed to assess the relative contributions of nitrification and denitrification to total N 2O emission. Results indicate: (1) nitrification was the primary source of N 2O emission, (2) cultivation increased nitrifier abundance but decreased nitrifier richness, (3) denitrifier abundance was not affected by cultivation but richness was increased by cultivation, and (4) differences in nitrifier and denitrifier communities composition and abundance between land-use and landform did not correspond to differences in N 2O emission.