Abstract
The present study assessed the effect of resveratrol on the expression of SIRT1 and mitochondrial quality and quantity in porcine oocytes. Supplementing the maturation medium with 20 µM resveratrol increased the expression of SIRT1, and enhanced mitochondrial functions, as observed from the increased ATP content and mitochondrial membrane potential. Addition of resveratrol also improved the ability of oocytes to develop into the blastocyst stage following activation. The effects of resveratrol on mitochondrial number were examined by comparing the mitochondrial DNA copy number (Mt number) between group of oocytes collected from the same donor gilt ovaries. Supplementing the maturation medium with only resveratrol did not affect the Mt number in the oocytes. However, supplementing the maturation medium with 10 µM MG132, a proteasome inhibitor, significantly increased the amount of ubiquitinated proteins and Mt number by 12 and 14%, respectively. In addition, when resveratrol was added to the medium containing MG132, the Mt number increased significantly by 39%, this effect was diminished by the addition of the SIRT1 inhibitor EX527. Furthermore, supplementing the medium with MG132 and EX527 did not affect Mt number. The mean SIRT1 expression in 20 oocytes was significantly and positively correlated with the Mt number in oocytes collected from the same donor. This study suggests that the expression of SIRT1 is associated with the Mt number in oocytes. In addition, activation of SIRT1 by resveratrol enhances the biosynthesis and degradation of mitochondria in oocytes, thereby replenishing and improving mitochondrial function and the developmental ability of oocytes.
Highlights
Mitochondria are multifunctional organelles that are important for energy production, apoptosis and calcium homeostasis
SIRT1 expression was significantly and positively correlated with the Mt number of oocytes collected from the same donor (r = 0.413; P,0.05, Figure 4). This investigation demonstrates for the first time that the addition of resveratrol to maturation medium upregulates the biosynthesis and degradation of mitochondria in porcine oocytes, which improves mitochondrial function and oocyte developmental competence
Active mitochondrial biogenesis has been reported in oocytes [15,27], little is known about mitochondrial homeostasis in these cells
Summary
Mitochondria are multifunctional organelles that are important for energy production, apoptosis and calcium homeostasis. We previously reported the kinetics of Mt number in bovine oocytes using a unique system where the predicted Mt number for a group of 10 oocytes closely resembled the Mt number of another group of 10 oocytes collected from the same donor [15]. Using this model, we can assess the effect of supplements in the maturation medium on the Mt number in oocytes. We examined the effect of resveratrol added to the maturation medium on SIRT1 expression, the developmental ability of oocytes, and mitochondrial functions in porcine oocytes. Results from this study reveal the relationship between SIRT1 and mitochondrial quality and quantity during oocyte maturation
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