Relationship between memory Tfh cells and unexplained recurrent spontaneous abortion

Abstract

Objective To discuss the relationship between memory Tfh cells and unexplained recurrent spontaneous abortion (URSA). Methods The peripheral blood and deciduas of 15 URSA patients were got, and 15 normal patients early pregnancy women were taken as the control. The CD4+CXCR5+memory Tfh cells and CD4+CXCR5+PD1+memory Tfh cells were assessed by flow cytometry. Results The CD4+CXCR5+memory Tfh cells and CD4+CXCR5+PD1+memory Tfh cells were both significantly increased in deciduas of the URSA patients compared with the control (P 0.05). Conclusion The up-regulation of decidual memory Tfh cells in human early pregnancy led to the failure of the pregnancy, abnormal activation of the immune maternal-fetal interface may cause the occurrence of URSA, which provides a basis for further study. Key words: Unexplained recurrent spontaneous abortion (URSA); Memory Tfh; Peripheral blood mononuclear cell (PBMC); Decidua; Flow cytometry (FCM)