Relationship between MEG3 gene polymorphism and risk of gastric cancer in Chinese population with high incidence of gastric cancer.

Xiaoling Kong,Geyu Liang,Hanqing Tang,Yingan Chen,Xiaomei Zhang,Yun Zhou,Caiping Liu,Sheng Yang

doi:10.1042/bsr20200305

Xiaoling Kong, Geyu Liang + Show 6 more

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https://doi.org/10.1042/bsr20200305

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Journal: Bioscience reports	Publication Date: Nov 24, 2020
Citations: 14	License type: CC BY 4.0

Affiliation: Southeast University, Jiangsu Cancer Hospital

Abstract

Objective: Gastric cancer is the most common gastrointestinal malignancy in China and results from a combination of genetic and environmental factors. The present study was conducted to investigate the relationship between long noncoding RNA (lncRNA) materally expressed gene 3 (MEG3) single nucleotide polymorphisms (SNPs) and the risk of gastric cancer and to construct a genetic-environmental risk assessment model. Methods: A case–control study was conducted to include 474 patients with gastric cancer diagnosed by clinical and pathological examination and 543 healthy physical examination subjects. Blood samples, general demographic data and behavioral lifestyle of the subjects were collected. The TaqMan real-time PCR method was used for testing the genotypes of MEG3 rs7158663 and rs10132552. Results: The A allele at the rs7158663 loci of MEG3 was found to be risk factor for gastric cancer (odds ratio (OR) = 1.41, 95% confidence interval (95% CI) = 1.14–1.74, P=0.002). Yet, no significant association between rs10132552 polymorphisms and gastric cancer was observed. Drinking, tea drinking and preserved food eating were risk factors for gastric cancer (P<0.05). A genetic–environmental risk assessment model was established by using the logistic regression model to include MEG3 rs7158663, drinking, tea drinking, and preserved food eating. With the increase in risk score (RS), the risk of gastric cancer increased substantially (P<0.05). And the area under the receiver operating characteristic (ROC) curve was 0.745, which indicates a high diagnostic value. Conclusions: MEG3 rs7158663 might be associated with the risk of gastric cancer; the diagnostic ability of genetic–environmental risk assessment model for gastric cancer is better.

Highlights

As one of the malignant tumors with high morbidity and mortality, gastric cancer is a serious threat to human health
The results showed that in the codominant model, GA genotype carriers had a 42% increased risk of gastric cancer compared with the GG genotype (OR = 1.42, 95% 95% confidence interval (CI): 1.06–1.90, P=0.020)
The results showed that the risk of developing the A allele was increased by 41% compared with the G allele (OR = 1.41, 95% confidence interval (95% CI): 1.14–1.74, P=0.002)

Summary

Introduction

As one of the malignant tumors with high morbidity and mortality, gastric cancer is a serious threat to human health. In China, gastric cancer is the most common malignant tumor of the digestive tract and its morbidity and mortality rate rank second among all malignant tumors [2]. High levels of carcinogens such as nitrites and polycyclic aromatic hydrocarbons in food are important causes of high incidence of gastric cancer in this area. There are few studies on the risk factors of Wuwei gastric cancer. Mi et al [4] showed that the genetic susceptibility factor accounted for approximately one-fifth to one-fourth in the formation of gastric cancer familial aggregation. The occurrence of gastric cancer is mainly the result of the License 4.0 (CC BY)

Methods

Results

Conclusion