Relationship between larval-pupal metamorphosis and gene expression of insulin-like peptide and insulin receptor in Spodoptera littoralis

Abstract

Event Abstract Back to Event Relationship between larval-pupal metamorphosis and gene expression of insulin-like peptide and insulin receptor in Spodoptera littoralis Masatoshi Iga1 and Guy Smagghe1* 1 Ghent University, Department of Crop Protection, Faculty of Bioscience Engineering, Belgium Insulin-like peptides (ILPs) affect a wide variety of biological events, such as metabolism, lifespan, growth and reproduction. Two ILPs (Sl-ILP1 and Sl-ILP2) were identified in the cotton leafworm, Spodoptera littoralis, while the functions and developmental characters are not fully understood. In the present study, we identified the partial sequence of the S. littoralis insulin receptor (Sl-InR) in addition to Sl-ILPs, and followed the expression profile of these genes during larval-pupal development. Sl-ILP1 and Sl-ILP2 were specifically expressed in brain, and their gene expressions were gradually decreased in concert with larval-pupal development. On the other hand, Sl-InR was expressed in all the selected tissues (brain, testis, fat body, Malpighian tubules, prothoracic glands, midgut), though the gene expression pattern was different among the tissues. Interestingly, the gene expression pattern of Sl-InR in fat body seemed to relate with larval-pupal development. In a parallel experiment, the juvenile hormone mimetic methoprene was able to prolong the larval period, and the gene expression of Sl-ILPs and Sl-InR was affected. These results clearly showed the relationship between gene expression of Sl-ILPs and larval-pupal development, and suggested the effect of Sl-ILPs may be controlled by not only Sl-ILPs expression but also Sl-InR expression. Keywords: comparative endocrinology Conference: 25th Conference of the European Comparative Endocrinologists, Pécs, Hungary, 31 Aug - 4 Sep, 2010. Presentation Type: Conference Presentation Topic: Comparative endocrinology Citation: Iga M and Smagghe G (2010). Relationship between larval-pupal metamorphosis and gene expression of insulin-like peptide and insulin receptor in Spodoptera littoralis. Front. Endocrinol. Conference Abstract: 25th Conference of the European Comparative Endocrinologists. doi: 10.3389/conf.fendo.2010.01.00061 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Aug 2010; Published Online: 29 Aug 2010. * Correspondence: Dr. Guy Smagghe, Ghent University, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent, Belgium, guysma9@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Masatoshi Iga Guy Smagghe Google Masatoshi Iga Guy Smagghe Google Scholar Masatoshi Iga Guy Smagghe PubMed Masatoshi Iga Guy Smagghe Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.