Relationship between Interleukin-10 Polymorphism and Maternal Serum Leptin Level in Preeclampsia

Abstract

The objective of this study is to show that pregnancy is a unique immune phenomenon because the feto-placental unit can develop without being attacked by the maternal immune system despite the admixing of maternal and fetal cells. There is a growing body of evidence that regulatory T cells (Tregs) act as modulators of vascular homeostasis. The aim of this study was to evaluate the role of interleukin-10 polymorphism along with leptin in the pathogenesis of preeclampsia. The study was carried out on 20 primigravida pregnant women with preeclampsia and 20 normal primigravida pregnant women. Blood samples sent for laboratory tests showed the presence of serum leptin, which was determined by DRGLeptin sandwich (Enzyme-linked immunosorbent assay (ELISA) technique) (EIA-2395; DRG International, Inc., Mountainside, NJ, USA) and serum IL-10 was determined by ELISA (Genzyme, Cambridge, MA, USA). The detection of IL-10 polymorphism was done by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques. The results of this study show that there was a significant difference between the frequency of genotype in patients and control group with regard to GG genotype, AA genotype, A allele, and G allele (P < .05) However, it was not significant with regard to the frequency of AG genotype (P > .05). The serum leptin was found to be significantly higher in preeclampsia group and with a more significant increase in AA genotype.