Relationship between human heat shock protein 60 IgG from porphyromonous gingivalis and coronary heart disease in the elderly

Abstract

Objective To investigate the relationships of human heat shock protein 60 (HSP 60), heat shock protein 60 IgG (HSP60 IgG), heat shock protein 60 IgG from porphyromonous gingivalis (P.g HSP60 IgG) with coronary heart disease and periodontitis, in order to provide the theoretical basis on the new risk factors for coronary heart disease. Methods A total of 128 veteran cadres who took health physical examination in the Second Hospital of Tianjin Medical University from September to October in 2012 were recruited. They were divided into 4 groups: the healthy control group (n=30), coronary heart disease group (CHD group, n=27), the periodontitis group (n=36), CHD combined with periodontitis group (n=35). The concentrations of HSP60, HSP60 IgG, P. g HSP60 IgG were determined by enzyme-linked immunosorbent assay (ELISA), and the general biochemical markers such as liver and kidney function, blood lipids were detected at the same time. Results The concentrations of human HSP60, HSP60 IgG, P. gHSP60 IgG were higher in CHD, periodontitis and CHD combined with periodontitis groups than in the healthy control group (all P<0.05). The above indicators in coronary heart disease combined with periodontitis group were also increased compared with those in CHD and periodontitis groups (all P<0.05). The patients with periodontitis had higher P. g HSP60 IgG concentration than patients with CHD (P<0.05). The partial correlation analysis showed that HSP60 and P. g Hsp60 IgG levels were positively correlated with human HSP60IgG level (r=0.705, 0.825, P<0.01). Multivariate logistic regression analysis showed that human HSP60, HSP60IgG and P. gHsp60IgG may be the risk factors for coronary heart disease (all P<0.05). Conclusions Human HSP60, HSP60 IgG and P. gHSP60 IgG may play a bridging role between coronary heart disease and periodontitis. The elderly patients with periodontitis may be more susceptible to coronary heart disease. Key words: Periodontitis; Coronary disease; Heat-shock proteins; Porphyromonas, endodontalis