Relationship between Helicobacter pylori infection and expressions of TERT and PCNA during gastric carcinomatous change

Abstract

Objective To investigate the expressions of cell proliferation of nuclear antigen (PCNA) and telomerase reverse transcriptase (TERT) mRNA and their clinical significance ingastri precance-rous lesion and carcinoma.To analyse the relationship between Helicobacter pylori infection and expressions of TERT and PCNA. Methods Expressions of Hp,TERT mRNA and proliferating cell nuclear antigen in 127 cases of different gastric pathological changes were respectively determined by in situ hybridization,Warthin-Starry and immunohistochemistry staining. Results The positive staining rates of TERT mRNA and PCNA in GC were respectively higher than those in gastric precancerous lesion group (P＜0.05). In Hp positive group, the expression of TERT mRNA and PCNA were higher than that in Hp negative group(P＜0.01). Conclusions Helicobacter pylori can stimulate gastric mucosa to excessive proliferate,at the same time,can induce the telomerase to activate and the disorder expression of PCNA, thereby, priming and accelerating the pathogenesis and development of gastric carcinoma.The excessive expression of PCNA and TERT are related to gastric carcinoma.Using them to screening high risk of precancerous lesion of gastric carcinoma. Key words: Gastric carcinoma ; Helicobacter pylori ; Cell proliferation of nuclear antigen ; Telomerase reverse transcriptase ;