Relationship Between Heart Damages and HSPs mRNA in Persistent Heat Stressed Broilers

Abstract

The relationship between myocardial cell damages and HSPs mRNA transcription in heat stressed broilers was studied using a spectrophotometer, the histopathological technique, and fluorescence quantitative reverse transcription PCR (FQ RT-PCR). The results showed that the activities of creatine kinase (CK) and glutamic-pyruvic transaminase (GPT) were induction during the persistent heat stress. The major lesions of the myocardial fibers were granular degeneration and necrosis. The transcription of constitutive or cognate heat shock protein 70 (HSC 70) mRNA was changeable. The transcription of heat shock protein 70 (HSP 70) mRNA was increased obviously in the course of persistent heat stress. The results showed that the change of HSC 70 mRNA transcription was contrary to the activity of CK, and the level of HSC 70 mRNA transcription must be used as a symbol of the myocardial cell damages in the course of persistent heat stress.