Relationship between gene polymorphism of beta 3-adrenergic receptor and diabetic foot

Abstract

Objective To explore the relationship between Trp64Arg polymorphism of the β3-adrenergic receptor(β3-AR) gene and diabetic foot in Chinese subjects.Methods The mutational genotype of Trp64Arg in β3-AR gene was measured by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 110 patients with type 2 diabetes mellitus (T2DM group) [(58 with diabetic foot ulcer (DFU group) and 52 without (N-DFU group)] and 42 normal healthy subjects (normal control group).The genotype (TT,TA or AA) and allele frequencies(T or A) were compared among groups.The anthro-pometry and metabolic indices were also compared between groups.Results (1) No significant deviation from Hardy-Weinberg equilibrium was found in the β3-AR Trp64Arg genotype frequency.In T2DM group,body mass index and homeostasis model assessment for insulin resistance index (HOMA-IR) in Arg allele were significantly higher than those without mutations allele (P =0.007 2,P=0.038 6).(2) In T2DM group,Arg64 carriers had lower level of high density lipoprotein-cholesterol than those without this mutation (P=0.047 5).(3)The frequency of Arg64 allele in DFU group was greatly higher than that of N-DFU group(x2=5.147,P＜0.05),while no difference in β3-AR gene genotype and allele frequency was observed between T2DM group and normal control group(x2=0.067,P ＞0.05).Conclusion β3-AR gene polymorphism is associated with diabetic foot.The Arg64 allele may be one of the risk factors of diabetic foot. Key words: Beta-3 adrenergic receptor; Gene polymorphism; Diabetic foot; Insulin resistance