Abstract

The electromotive force (EMF) occurring during batch culture of an iron-oxidizing bacterium was measured, and the relationship between the time evolution of the EMF, substrate concentration and bacterial density was investigated both experimentally and theoretically. The aim was to examine whether the monitoring and control of the culture process by measurement of the EMP and the utilization of electrical energy were possible. The measured and theoretical maximum EMF values were 288 mV and 322 mV respectively. The EMF calculated using the Nernst equation, based on the Monod equation of bacterial growth and the theoretical time course of the consumption of limiting substrate (FeSO 4), was in good agreement with the measured values.

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