Relationship between down-regulation of LRIGI expression and growth of glioma cells

Abstract

Objective To investigate the interaction between LRIG1 gene and epidermal growth factor receptor (EGFR) in human glioma cells and explore the action mechanism of effects of the two genes on growth of glioma cells. Methods Co-immunoprecipitation was performed to detect the interaction of LRIGI and EGFR in human gtioma cell line GL15. Small interfering RNA (siRNA) targeting LRIG1 gene and negative shRNA (neg) were constructed and transfected into GLI5 glioma cells. And the cells (siRNA) that stably suppressed LRIG1 expression were selected by G418 (600 mg/L). The change of LRIG1 protein level was measured by Western blot,and growth curves were determined by MTT assay. Re-suits The interaction of LRIG1 and EGFR in human glioma cell line GL15 was confirmed by co-immuno-precipitation, siRNA targeting LRIGI gene and negative shRNA (neg) were successfully constructed. The LRIG1 protein level in pGenesi 12-LRIG1-shRNA (siRNA) transfected cells was significantly silenced by 54.7% ,and the EGFR protein level increased by 57.1%. The siRNA cells had higher proliferation rate than controls. Conclusion The LRIG1 protein inhibits tumor generation and proliferation by interacting with EGFR,which was weakened by interfering LRIG1 expression,leading to the promotion of tumor prolif eration. Key words: Glioma; Epidermal growth factor receptor; RNAi; Cell proliferation