Abstract
The rate of incorporation of acetate into cholesterol by rat liver slices and the rates of 7 α-hydroxylation of cholesterol, of 12 α-hydroxylation of 7 α-hydroxy-4-cholesten-3-one and of 7 α-hydroxylation of taurodeoxycholic acid by rat liver microsomes were assayed at six different times of the day. The diurnal variations in rate of incorporation of acetate into cholesterol (maximum between 8 p.m. and midnight and minimum at noon) and in rate of 7 α-hydroxylation of cholesterol (maximum at 8 p.m. and minimum at noon) followed each other closely. 12 α-Hydroxylation of 7 α-hydroxy-4-cholesten-3-one and 7 α-hydroxylation of taurodeoxycholic acid remained at about the same level during the day. The pattern of diurnal variations in incorporation of acetate into cholesterol and in 7 α-hydroxylation of cholesterol was changed by reversing the daily rhythm of the rats. The results indicate that changes in rates of biosynthesis of cholesterol and bile acids occur practically concomitantly.
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