Relationship between delayed luminescence emission and mitochondrial status in Saccharomyces cerevisiae

Miao Tian,Xiaochun Zhang,Peng Gao,Hong Wu,Qing Tang,Yanpeng Zhao,Yang Liu,Bing Wang,Chenhao Li,Qing Li,Danyu Li,Peng Zheng,Jing Wang

doi:10.1038/s41598-021-04290-9

Abstract

Delayed luminescence (DL) is gradually used in various detection of biological systems as a rapid detection technique, however, its biological mechanism was still not clear. In this study, a new model of DL detection system for liquid biological samples is established to investigate the DL emission of Saccharomyces cerevisiae cells cultured in different glucose concentrations. We analyzed the relationship between the DL emission and cell growth, cell vitality, mitochondrial morphology, mitochondrial DNA (mtDNA) copy number, adenosine triphosphate (ATP), oxygen consumption rate (OCR), as well as mitochondria membrane potential (MMP) in S. cerevisiae cells cultured with 0.01, 0.05, 0.15, 3, 10 and 20 g/L glucose respectively. It was found that the DL emission had strong correlation with mitochondrial morphology, OCR, and MMP. The results suggested that DL is an indicator of mitochondria status under different glucose supply conditions, and may be an effective method to detect mitochondrial metabolism related disorders.

Highlights

Delayed luminescence (DL) is gradually used in various detection of biological systems as a rapid detection technique, its biological mechanism was still not clear
The DL emitted from yeast cells which cultured with 0.01, 0.05, 0.15, 3, 10 and 20 g/L glucose respectively for 3 h was recorded after 405 nm laser excitation and evaluated in ENN software
It appeared that glucose concentration significantly affected the DL emission of yeast cells

Summary

Introduction

Delayed luminescence (DL) is gradually used in various detection of biological systems as a rapid detection technique, its biological mechanism was still not clear. A new model of DL detection system for liquid biological samples is established to investigate the DL emission of Saccharomyces cerevisiae cells cultured in different glucose concentrations. The results suggested that DL is an indicator of mitochondria status under different glucose supply conditions, and may be an effective method to detect mitochondrial metabolism related disorders. Mitochondria are the main sites of cellular oxidative respiration, the morphology, quantity and activity of mitochondria are directly affected by different fermentable carbohydrates concentrations. Because of this feature, S. cerevisiae is a good candidate to study the relationship between mitochondrial status and DL. A DL detection system that suitable for liquid biological sample was established and for the first time, to our knowledge, the correlation between DL emission and the glucose-induced mitochondrial characteristics was studied

Methods

Results

Conclusion