Relationship between chronic stress and consumption of Sweeteners in CD1 Mice

Abstract

The sweetener consumption and exposure to stress has increased with the current lifestyle, but this relationship has been little studied. The objective of this study was to evaluate the effect of the ingestion of sweeteners and exposure to stress on water and food consumption. We used forty 21‐days old CD1 mice, divided into 5 groups: Control without stress (CL), Control plus stress (CL + S), Sucrose plus stress (Suc + S), Sucralose plus stress (Sucral + S) and Stevia plus stress (ST+ S). They were identified body weight (BW), body mass index (BMI) taking the nose‐anus length (cm) and blood glucose (mg/dL). The sweetener was administered with micropipette everyday (4.1 mg/ml/g BW); the stress model was immobilization two hours daily (Monday to Sunday). Water consumption (without sweetener) and food is quantified for 24h per mouse. In the BMI, no difference was found between groups at the end of the study (F=1.598, p<0.184). The blood glucose concentration increased significantly in the group of Suc + S (F=3.70, p<0.008). Water consumption showed differences between groups (F=2.622, p<0.05), it was significantly elevated in the CL + S group and decreased in the Suc + S and Sucral + S groups. The feed intake showed difference between groups (F =26.72, p<0.001), was significantly lower in the CL + S, Suc + S and ST + S groups. Stress reduces food consumption, increased water consumption in sucrose and sucralose groups therefore the combination of stress and sweetener consumption maybe increases satiety.Support or Funding InformationUAEM