Relationship between chemotaxis and complement activation by ceramic biomaterials

Abstract

This paper examines the relationship between complement activation by ceramic biomaterials and chemotaxis. Complement activation was examined by both neutrophil polarization (a technique which has previously been used to examine chemotaxis) and immunoelectrophoresis assays. The results suggest that at increasing serum concentrations of both calcium hydrogen phosphate and coral (calcium carbonate) powder, the quantity of C3 activation increased, as did the quantity of serum-derived chemotactic factors. In the case of tricalcium phosphate powder, the quantity of C3 activation and the neutrophil polarization response to serum were similar for serum levels between 20 and 80 mg/ml. Complement C3 was not activated in serum incubated with calcium hydrogen phosphate powder and serum incubated with this material was not chemotactic for neutrophils.